A Small-Volume, Low-Cost, and Versatile Continuous Culture Device.

ABSTRACT:

Background

Continuous culture devices can be used for various purposes such as establishing reproducible growth conditions or maintaining cell populations under a constant environment for long periods. However, commercially available instruments are expensive, were not designed to handle small volumes in the milliliter range, and can lack the flexibility required for the diverse experimental needs found in several laboratories.

Methodology/principal findings

We developed a versatile continuous culture system and provide detailed instructions as well as a graphical user interface software for potential users to assemble and operate their own instrument. Three culture chambers can be controlled simultaneously with the proposed configuration, and all components are readily avai

SUBMITTER: Matteau D 

PROVIDER: S-EPMC4510131 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature