DNA Microarray-Based Identification of Genes Regulated by NtrC in Bradyrhizobium japonicum.

ABSTRACT: The Bradyrhizobium japonicum NtrBC two-component system is a critical regulator of cellular nitrogen metabolism, including the acquisition and catabolism of nitrogenous compounds. To better define the roles of this system, genome-wide transcriptional profiling was performed to identify the NtrC regulon during the response to nitrogen limitation. Upon cells perceiving low intracellular nitrogen, they stimulate the phosphorylation of NtrC, which induces genes responsible for alteration of the core glutamine synthetase/glutamate synthase nitrogen assimilation pathway, including the genes for the glutamine synthetases and PII proteins. In addition, genes responsible for the import and utilization of multiple nitrogen sources, specifically nitrate and nitrite, were upregulated by NtrC activatio

SUBMITTER: Franck WL 

PROVIDER: S-EPMC4510186 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature