Influence of Sex on Basal and Dickkopf-1 Regulated Gene Expression in the Bovine Morula.

ABSTRACT: Sex affects function of the developing mammalian embryo as early as the preimplantation period. There were two goals of the current objective. The first was to determine the degree and nature of differences in gene expression between female and male embryos in the cow at the morula stage of development. The second objective was to determine whether DKK1, a molecule known to alter differentiation of the blastocyst, would affect gene expression differently for female and male morulae. In Experiment 1, female and male embryos were treated with DKK1 at Day 5 after insemination. Morulae were harvested 24 h after treatment, pooled in groups of 20 for microarray analysis and RNA subjected to analysis of gene expression by microarray hybridization. There were 662 differentially expressed genes bet

SUBMITTER: Denicol AC 

PROVIDER: S-EPMC4510475 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature