A Stem Cell-Derived Platform for Studying Single Synaptic Vesicles in Dopaminergic Synapses.

ABSTRACT: The exocytotic release of dopamine is one of the most characteristic but also one of the least appreciated processes in dopaminergic neurotransmission. Fluorescence imaging has yielded rich information about the properties of synaptic vesicles and the release of neurotransmitters in excitatory and inhibitory neurons. In contrast, imaging-based studies for in-depth understanding of synaptic vesicle behavior in dopamine neurons are lagging largely because of a lack of suitable preparations. Midbrain culture has been one of the most valuable preparations for the subcellular investigation of dopaminergic transmission; however, the paucity and fragility of cultured dopaminergic neurons limits their use for live cell imaging. Recent developments in stem cell technology have led to the successful

SUBMITTER: Gu H 

PROVIDER: S-EPMC4511147 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature