Performing discovery-driven neonatal research by transcriptomic analysis of routinely discarded biofluids.

ABSTRACT:

Objective

To perform discovery-driven research on the neonatal salivary and cord blood transcriptomes.

Methods

Two separate cohorts of infants were enrolled in this study. In one, cord blood (n = 10) and in the other, saliva samples (n = 10) were collected at term gestation. Total RNA was extracted, amplified and hybridized onto Affymetrix HG U133a gene expression microarrays. Following normalization, genes expressed in the highest quintile (≥ 80%) across all subjects in each biofluid were analyzed with Ingenuity Pathway Analysis. Over-represented pathways relating to organ specific development and physiological functions in the newborn were explored.

Results

There were 303 genes in neonatal saliva and 282 genes in umbilical cord blood that met statistical criteria. O

SUBMITTER: Maron JL 

PROVIDER: S-EPMC4511475 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature