Large-scale contamination of microbial isolate genomes by Illumina PhiX control.

ABSTRACT: With the rapid growth and development of sequencing technologies, genomes have become the new go-to for exploring solutions to some of the world's biggest challenges such as searching for alternative energy sources and exploration of genomic dark matter. However, progress in sequencing has been accompanied by its share of errors that can occur during template or library preparation, sequencing, imaging or data analysis. In this study we screened over 18,000 publicly available microbial isolate genome sequences in the Integrated Microbial Genomes database and identified more than 1000 genomes that are contaminated with PhiX, a control frequently used during Illumina sequencing runs. Approximately 10% of these genomes have been published in literature and 129 contaminated genomes were sequen

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC4511556 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature