A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases.

ABSTRACT: A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

SUBMITTER: Angelini LM 

PROVIDER: S-EPMC4512080 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature