Protein Mobility Shifts Contribute to Gel Electrophoresis Liquid Chromatography Analysis.

ABSTRACT: Profiling of cellular and subcellular proteomes by liquid chromatography with tandem mass spectrometry (MS) after fractionation by SDS-PAGE is referred to as GeLC (gel electrophoresis liquid chromatography)-MS. The GeLC approach decreases complexity within individual MS analyses by size fractionation with SDS-PAGE. SDS-PAGE is considered an excellent fractionation technique for intact proteins because of good resolution for proteins of all sizes, isoelectric points, and hydrophobicities. Additional information derived from the mobility of the intact proteins is available after an SDS-PAGE fractionation, but that information is usually not incorporated into the proteomic analysis. Any chemical or proteolytic modification of a protein that changes the mobility of that protein in the gel can

SUBMITTER: Carruthers NJ 

PROVIDER: S-EPMC4512738 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature