Deciphering the Regulatory Circuitry That Controls Reversible Lysine Acetylation in Salmonella enterica.

ABSTRACT:

Unlabelled

In Salmonella enterica, the reversible lysine acetylation (RLA) system is comprised of the protein acetyltransferase (Pat) and sirtuin deacetylase (CobB). RLA controls the activities of many proteins, including the acetyl coenzyme A (acetyl-CoA) synthetase (Acs), by modulating the degree of Acs acetylation. We report that IolR, a myo-inositol catabolism repressor, activates the expression of genes encoding components of the RLA system. In vitro evidence shows that the IolR protein directly regulates pat expression. An iolR mutant strain displayed a growth defect in minimal medium containing 10 mM acetate, a condition under which RLA function is critical to control Acs activity. Increased levels of Pat, CobB, or Acs activity reversed the growth defect, suggesting the Pat/

SUBMITTER: Hentchel KL 

PROVIDER: S-EPMC4513076 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature