Engineering a Nickase on the Homing Endonuclease I-DmoI Scaffold.

ABSTRACT: Homing endonucleases are useful tools for genome modification because of their capability to recognize and cleave specifically large DNA targets. These endonucleases generate a DNA double strand break that can be repaired by the DNA damage response machinery. The break can be repaired by homologous recombination, an error-free mechanism, or by non-homologous end joining, a process susceptible to introducing errors in the repaired sequence. The type of DNA cleavage might alter the balance between these two alternatives. The use of "nickases" producing a specific single strand break instead of a double strand break could be an approach to reduce the toxicity associated with non-homologous end joining by promoting the use of homologous recombination to repair the cleavage of a single DNA brea

SUBMITTER: Molina R 

PROVIDER: S-EPMC4513113 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature