Histidine 114 Is Critical for ATP Hydrolysis by the Universally Conserved ATPase YchF.

ABSTRACT: GTPases perform a wide range of functions, ranging from protein synthesis to cell signaling. Of all known GTPases, only eight are conserved across all three domains of life. YchF is one of these eight universally conserved GTPases; however, its cellular function and enzymatic properties are poorly understood. YchF differs from the classical GTPases in that it has a higher affinity for ATP than for GTP and is a functional ATPase. As a hydrophobic amino acid-substituted ATPase, YchF does not possess the canonical catalytic Gln required for nucleotide hydrolysis. To elucidate the catalytic mechanism of ATP hydrolysis by YchF, we have taken a two-pronged approach combining classical biochemical and in silico techniques. The use of molecular dynamics simulations allowed us to complement our bio

SUBMITTER: Rosler KS 

PROVIDER: S-EPMC4513122 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature