Platelet geometry sensing spatially regulates α-granule secretion to enable matrix self-deposition.

ABSTRACT: Although the biology of platelet adhesion on subendothelial matrix after vascular injury is well characterized, how the matrix biophysical properties affect platelet physiology is unknown. Here we demonstrate that geometric orientation of the matrix itself regulates platelet α-granule secretion, a key component of platelet activation. Using protein microcontact printing, we show that platelets spread beyond the geometric constraints of fibrinogen or collagen micropatterns with <5-µm features. Interestingly, α-granule exocytosis and deposition of the α-granule contents such as fibrinogen and fibronectin were primarily observed in those areas of platelet extension beyond the matrix protein micropatterns. This enables platelets to "self-deposit" additional matrix, provide more cellular membra

SUBMITTER: Sakurai Y 

PROVIDER: S-EPMC4513253 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature