Quantitation of permethylated N-glycans through multiple-reaction monitoring (MRM) LC-MS/MS.

ABSTRACT: The important biological roles of glycans and their implications in disease development and progression have created a demand for the development of sensitive quantitative glycomics methods. Quantitation of glycans existing at low abundance is still analytically challenging. In this study, an N-linked glycans quantitation method using multiple-reaction monitoring (MRM) on a triple quadrupole instrument was developed. Optimum normalized collision energy (CE) for both sialylated and fucosylated N-glycan was determined to be 30%, whereas it was found to be 35% for either fucosylated or sialylated N-glycans. The optimum CE for mannose and complex type N-glycan was determined to be 35%. Additionally, the use of three transitions was shown to facilitate reliable quantitation. A total of 88 N-gly

SUBMITTER: Zhou S 

PROVIDER: S-EPMC4514032 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature