Obif, a Transmembrane Protein, Is Required for Bone Mineralization and Spermatogenesis in Mice.

ABSTRACT:

Background

Various kinds of transmembrane and secreted proteins play pivotal roles in development through cell-cell communication. We previously reported that Obif (Osteoblast induction factor, Tmem119), encoding a single transmembrane protein, is expressed in differentiating osteoblasts, and that Obif-/- mice exhibit significantly reduced bone volume in the femur. In the current study, we characterized the Obif protein and further investigated the biological phenotypes of a variety of tissues in Obif-/- mice.

Results

First, we found that O-glycosylation of the Obif protein occurs at serine residue 36 in the Obif extracellular domain. Next, we observed that Obif-/- mice exhibit bone dysplasia in association with significantly increased osteoid volume per osteoid surface (OV/

SUBMITTER: Mizuhashi K 

PROVIDER: S-EPMC4514473 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature