Comparison of Depletion Strategies for the Enrichment of Low-Abundance Proteins in Urine.

ABSTRACT: Proteome analysis of complex biological samples for biomarker identification remains challenging, among others due to the extended range of protein concentrations. High-abundance proteins like albumin or IgG of plasma and urine, may interfere with the detection of potential disease biomarkers. Currently, several options are available for the depletion of abundant proteins in plasma. However, the applicability of these methods in urine has not been thoroughly investigated. In this study, we compared different, commercially available immunodepletion and ion-exchange based approaches on urine samples from both healthy subjects and CKD patients, for their reproducibility and efficiency in protein depletion. A starting urine volume of 500 μL was used to simulate conditions of a multi-institutio

SUBMITTER: Filip S 

PROVIDER: S-EPMC4514849 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature