Common and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish.

ABSTRACT:

Background

Although the responses to many pathogen associated molecular patterns (PAMPs) in cell cultures and extracted organs are well characterized, there is little known of transcriptome responses to PAMPs in whole organisms. To characterize this in detail, we have performed RNAseq analysis of responses of zebrafish embryos to injection of PAMPs in the caudal vein at one hour after exposure. We have compared two ligands that in mammals have been shown to specifically activate the TLR2 and TLR5 receptors: Pam3CSK4 and flagellin, respectively.

Results

We identified a group of 80 common genes that respond with high stringency selection to stimulations with both PAMPs, which included several well-known immune marker genes such as il1b and tnfa. Surprisingly, we also identifie

SUBMITTER: Yang S 

PROVIDER: S-EPMC4514945 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature