GTSE1 expression represses apoptotic signaling and confers cisplatin resistance in gastric cancer cells.

ABSTRACT:

Background

Platinum based therapy is commonly used in the treatment of advanced gastric cancer. However, resistance to chemotherapy is a major challenge that causes marked variation in individual response rate and survival rate. In this study, we aimed to identify the expression of GTSE1 and its correlation with cisplatin resistance in gastric cancer cells.

Methods

Methylation profiling was carried out in tissue samples from gastric cancer patients before undergoing neoadjuvent therapy using docetaxel, cisplatin and 5FU (DCX) and in gastric cancer cell lines. The correlation between GTSE1 expression and methylation in gastric cancer cells was determined by RT-PCR and MSP respectively. GTSE1 expression was knocked-down using shRNA's and its effects on cisplatin cytotoxicity a

SUBMITTER: Subhash VV 

PROVIDER: S-EPMC4514980 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature