The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes.

ABSTRACT: Enzymes capable of inactivating tetracycline are paradoxically rare compared with enzymes that inactivate other natural-product antibiotics. We describe a family of flavoenzymes, previously unrecognizable as resistance genes, which are capable of degrading tetracycline antibiotics. From soil functional metagenomic selections, we discovered nine genes that confer high-level tetracycline resistance by enzymatic inactivation. We also demonstrate that a tenth enzyme, an uncharacterized homolog in the human pathogen Legionella longbeachae, similarly inactivates tetracycline. These enzymes catalyze the oxidation of tetracyclines in vitro both by known mechanisms and via previously undescribed activity. Tetracycline-inactivation genes were identified in diverse soil types, encompass substantial s

SUBMITTER: Forsberg KJ 

PROVIDER: S-EPMC4515146 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature