Combined Whole Methylome and Genomewide Association Study Implicates CNTN4 in Alcohol Use.

ABSTRACT:

Background

Methylome-wide association (MWAS) studies present a new way to advance the search for biological correlates for alcohol use. A challenge with methylation studies of alcohol involves the causal direction of significant methylation-alcohol associations. One way to address this issue is to combine MWAS data with genomewide association study (GWAS) data.

Methods

Here, we combined MWAS and GWAS results for alcohol use from 619 individuals. Our MWAS data were generated by next-generation sequencing of the methylated genomic DNA fraction, producing over 60 million reads per subject to interrogate methylation levels at ~27 million autosomal CpG sites in the human genome. Our GWAS included 5,571,786 single nucleotide polymorphisms (SNPs) imputed with 1000 Genomes.

Resul

SUBMITTER: Clark SL 

PROVIDER: S-EPMC4515164 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature