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Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin.

ABSTRACT: Laser-scanning cytometry is presented as a tool allowing population scale analysis of ex vivo human brown adipogenic differentiation. It combines texture analysis and detection of Ucp1 protein content in single brown adipocytes of mixed cell populations with gene expression pattern and functional characteristics of browning. Using this method we could validate mouse data in human samples demonstrating the effectiveness of irisin to induce "beige" differentiation of subcutaneous white adipocytes.

SUBMITTER: Kristof E 

PROVIDER: S-EPMC4515591 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin.

Kristóf Endre E   Doan-Xuan Quang-Minh QM   Bai Péter P   Bacso Zsolt Z   Fésüs László L  

Scientific reports 20150727

Laser-scanning cytometry is presented as a tool allowing population scale analysis of ex vivo human brown adipogenic differentiation. It combines texture analysis and detection of Ucp1 protein content in single brown adipocytes of mixed cell populations with gene expression pattern and functional characteristics of browning. Using this method we could validate mouse data in human samples demonstrating the effectiveness of irisin to induce "beige" differentiation of subcutaneous white adipocytes. ...[more]

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