Amplicon sequencing for the quantification of spoilage microbiota in complex foods including bacterial spores.

ABSTRACT:

Background

Spoilage of food products is frequently caused by bacterial spores and lactic acid bacteria. Identification of these organisms by classic cultivation methods is limited by their ability to form colonies on nutrient agar plates. In this study, we adapted and optimized 16S rRNA amplicon sequencing for quantification of bacterial spores in a canned food matrix and for monitoring the outgrowth of spoilage microbiota in a ready-to-eat food matrix.

Results

The detection limit of bar-coded 16S rRNA amplicon sequencing was determined for the number of bacterial spores in a canned food matrix. Analysis of samples from a canned food matrix spiked with a mixture of equinumerous spores from the thermophiles, Geobacillus stearothermophilus and Geobacillus thermoglucosidans, an

SUBMITTER: de Boer P 

PROVIDER: S-EPMC4515881 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature