Development of Animal Models of Local Retinal Degeneration.

ABSTRACT:

Purpose

Development of nongenetic animal models of local retinal degeneration is essential for studies of retinal pathologies, such as chronic retinal detachment or age-related macular degeneration. We present two different methods to induce a highly localized retinal degeneration with precise onset time, that can be applied to a broad range of species in laboratory use.

Methods

A 30-μm thin polymer sheet was implanted subretinally in wild-type (WT) rats. The effects of chronic retinal separation from the RPE were studied using histology and immunohistochemistry. Another approach is applicable to species with avascular retina, such as rabbits, where the photoreceptors and RPE were thermally ablated over large areas, using a high power scanning laser.

Results

Photorece

SUBMITTER: Lorach H 

PROVIDER: S-EPMC4516014 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature