Genomic alterations in head and neck squamous cell carcinoma determined by cancer gene-targeted sequencing.

ABSTRACT:

Background

To determine genomic alterations in head and neck squamous cell carcinoma (HNSCC) using formalin-fixed, paraffin-embedded (FFPE) tumors obtained through routine clinical practice, selected cancer-related genes were evaluated and compared with alterations seen in frozen tumors obtained through research studies.

Patients and methods

DNA samples obtained from 252 FFPE HNSCC were analyzed using next-generation sequencing-based (NGS) clinical assay to determine sequence and copy number variations in 236 cancer-related genes plus 47 introns from 19 genes frequently rearranged in cancer. Human papillomavirus (HPV) status was determined by presence of the HPV DNA sequence in all samples and corroborated with high-risk HPV in situ hybridization (ISH) and p16 immunohistoche

SUBMITTER: Chung CH 

PROVIDER: S-EPMC4516044 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature