Heparin enhances uptake of platelet factor 4/heparin complexes by monocytes and macrophages.

ABSTRACT:

Background

Heparin-induced thrombocytopenia (HIT) is an iatrogenic complication of heparin therapy caused by antibodies to a self-antigen, platelet factor (4) and heparin. The reasons why antibodies form to PF4/heparin, but not to PF4 bound to other cellular glycosaminoglycans are poorly understood.

Objective

To investigate differences in cellular responses to cell-bound PF4 and PF4/heparin complexes, we studied the internalization of each by peripheral blood-derived monocytes, dendritic cells and neutrophils.

Methods and results

Using unlabeled and fluorescently-labeled antigen and/or labeled monoclonal antibody to PF4/heparin complexes (KKO), we show that PF4/heparin complexes are taken up by monocytes in a heparin-dependent manner and are internalized by human mono

SUBMITTER: Joglekar M 

PROVIDER: S-EPMC4516590 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature