Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single fluorescent biosensor.

ABSTRACT: Mitochondrial dysfunction is implicated in many human diseases and occurs in normal aging. Mitochondrial health is maintained through organelle biogenesis and repair or turnover of existing mitochondria. Mitochondrial turnover is principally mediated by mitophagy, the trafficking of damaged mitochondria to lysosomes via macroautophagy (autophagy). Mitophagy requires autophagy, but is itself a selective process that relies on specific autophagy-targeting mechanisms, and thus can be dissociated from autophagy under certain circumstances. Therefore, it is important to assess autophagy and mitophagy together and separately. We sought to develop a robust, high-throughput, quantitative method for monitoring both processes in parallel. Here we report a flow cytometry-based assay capable of rapid

SUBMITTER: Sargsyan A 

PROVIDER: S-EPMC4517063 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature