Ultra Deep Sequencing of a Baculovirus Population Reveals Widespread Genomic Variations.

ABSTRACT: Viruses rely on widespread genetic variation and large population size for adaptation. Large DNA virus populations are thought to harbor little variation though natural populations may be polymorphic. To measure the genetic variation present in a dsDNA virus population, we deep sequenced a natural strain of the baculovirus Autographa californica multiple nucleopolyhedrovirus. With 124,221X average genome coverage of our 133,926 bp long consensus, we could detect low frequency mutations (0.025%). K-means clustering was used to classify the mutations in four categories according to their frequency in the population. We found 60 high frequency non-synonymous mutations under balancing selection distributed in all functional classes. These mutants could alter viral adaptation dynamics, either t

SUBMITTER: Chateigner A 

PROVIDER: S-EPMC4517117 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature