Analysis of IS6110 insertion sites provide a glimpse into genome evolution of Mycobacterium tuberculosis.

ABSTRACT: Insertion sequence (IS) 6110 is found at multiple sites in the Mycobacterium tuberculosis genome and displays a high degree of polymorphism with respect to copy number and insertion sites. Therefore, IS6110 is considered to be a useful molecular marker for diagnosis and strain typing of M. tuberculosis. Generally IS6110 elements are identified using experimental methods, useful for analysis of a limited number of isolates. Since short read genome sequences generated using next-generation sequencing (NGS) platforms are available for a large number of isolates, a computational pipeline for identification of IS6110 elements from these datasets was developed. This study shows results from analysis of NGS data of 1377 M. tuberculosis isolates. These isolates represent all seven major global lin

SUBMITTER: Roychowdhury T 

PROVIDER: S-EPMC4517164 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature