Ontology highlight
ABSTRACT:
SUBMITTER: Li W
PROVIDER: S-EPMC4517844 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Li Wen W Liu Zheng Z Koripella Ravi Kiran RK Langlois Robert R Sanyal Suparna S Frank Joachim J
Science advances 20150501 4
During protein synthesis, elongation of the polypeptide chain by each amino acid is followed by a translocation step in which mRNA and transfer RNA (tRNA) are advanced by one codon. This crucial step is catalyzed by elongation factor G (EF-G), a guanosine triphosphatase (GTPase), and accompanied by a rotation between the two ribosomal subunits. A mutant of EF-G, H91A, renders the factor impaired in guanosine triphosphate (GTP) hydrolysis and thereby stabilizes it on the ribosome. We use cryogeni ...[more]