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A Major Role of DNA Polymerase ? in Replication of Both the Leading and Lagging DNA Strands.

ABSTRACT: Genetic studies with S. cerevisiae Pol? (pol3-L612M) and Pol? (pol2-M644G) mutant alleles, each of which display a higher rate for the generation of a specific mismatch, have led to the conclusion that Pol? is the primary leading strand replicase and that Pol? is restricted to replicating the lagging strand template. Contrary to this widely accepted view, here we show that Pol? plays a major role in the replication of both DNA strands, and that the paucity of pol3-L612M-generated errors on the leading strand results from their more proficient removal. Thus, the apparent lack of Pol? contribution to leading strand replication is due to differential mismatch removal rather than differential mismatch generation. Altogether, our genetic studies with Pol3 and Pol2 mutator alleles support the conclusion that Pol?, and not Pol?, is the major DNA polymerase for carrying out both leading and lagging DNA synthesis.

SUBMITTER: Johnson RE 

PROVIDER: S-EPMC4517859 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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A Major Role of DNA Polymerase δ in Replication of Both the Leading and Lagging DNA Strands.

Johnson Robert E RE   Klassen Roland R   Prakash Louise L   Prakash Satya S  

Molecular cell 20150702 2

Genetic studies with S. cerevisiae Polδ (pol3-L612M) and Polε (pol2-M644G) mutant alleles, each of which display a higher rate for the generation of a specific mismatch, have led to the conclusion that Polε is the primary leading strand replicase and that Polδ is restricted to replicating the lagging strand template. Contrary to this widely accepted view, here we show that Polδ plays a major role in the replication of both DNA strands, and that the paucity of pol3-L612M-generated errors on the l  ...[more]

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