Transcriptional analysis of susceptible and resistant European corn borer strains and their response to Cry1F protoxin.

ABSTRACT:

Background

Despite a number of recent reports of insect resistance to transgenic crops expressing insecticidal toxins from Bacillus thuringiensis (Bt), little is known about the mechanism of resistance to these toxins. The purpose of this study is to identify genes associated with the mechanism of Cry1F toxin resistance in European corn borer (Ostrinia nubilalis Hübner). For this, we compared the global transcriptomic response of laboratory selected resistant and susceptible O. nubilalis strain to Cry1F toxin. We further identified constitutive transcriptional differences between the two strains.

Results

An O. nubilalis midgut transcriptome of 36,125 transcripts was assembled de novo from 106 million Illumina HiSeq and Roche 454 reads and used as a reference for estimation o

SUBMITTER: Nanoth Vellichirammal N 

PROVIDER: S-EPMC4518661 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature