The Protein Interactome of Mycobacteriophage Giles Predicts Functions for Unknown Proteins.

ABSTRACT:

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Mycobacteriophages are viruses that infect mycobacterial hosts and are prevalent in the environment. Nearly 700 mycobacteriophage genomes have been completely sequenced, revealing considerable diversity and genetic novelty. Here, we have determined the protein complement of mycobacteriophage Giles by mass spectrometry and mapped its genome-wide protein interactome to help elucidate the roles of its 77 predicted proteins, 50% of which have no known function. About 22,000 individual yeast two-hybrid (Y2H) tests with four different Y2H vectors, followed by filtering and retest screens, resulted in 324 reproducible protein-protein interactions, including 171 (136 nonredundant) high-confidence interactions. The complete set of high-confidence interactions among Giles proteins

SUBMITTER: Mehla J 

PROVIDER: S-EPMC4518834 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature