ITRAQ-based proteomic profiling of Vibrio parahaemolyticus under various culture conditions.

ABSTRACT:

Background

Vibrio parahaemolyticus is a common pathogen infecting humans and marine animals; this pathogen has become a major concern of marine food products and trade. In this study, V. parahaemolyticus isolated from sewage was exposed to different culture conditions and analyzed by isobaric tag for relative and absolute quantitation (iTRAQ) based reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique. Our goal is to gain further insights into the proteomics of V. parahaemolyticus, particularly differentially expressed proteins closely correlated with growth conditions and pathogenicity associated proteins.

Results

In this study, a total of 2,717 proteins including numerous membrane proteins were significantly identified, and 616 proteins display

SUBMITTER: Yang W 

PROVIDER: S-EPMC4518887 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature