Robustness of Next Generation Sequencing on Older Formalin-Fixed Paraffin-Embedded Tissue.

ABSTRACT: Next Generation Sequencing (NGS) technologies are used to detect somatic mutations in tumors and study germ line variation. Most NGS studies use DNA isolated from whole blood or fresh frozen tissue. However, formalin-fixed paraffin-embedded (FFPE) tissues are one of the most widely available clinical specimens. Their potential utility as a source of DNA for NGS would greatly enhance population-based cancer studies. While preliminary studies suggest FFPE tissue may be used for NGS, the feasibility of using archived FFPE specimens in population based studies and the effect of storage time on these specimens needs to be determined. We conducted a study to determine whether DNA in archived FFPE high-grade ovarian serous adenocarcinomas from Surveillance, Epidemiology and End Results (SEER) reg

SUBMITTER: Carrick DM 

PROVIDER: S-EPMC4519244 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature