Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes.

ABSTRACT:

Objective

To examine the genome-wide distribution of hydroxymethylated cytosine (5hmC) in osteoarthritic (OA) and normal chondrocytes in order to investigate the effect on OA-specific gene expression.

Methods

Cartilage was obtained from OA patients undergoing total knee arthroplasty or from control patients undergoing anterior cruciate ligament reconstruction. Genome-wide sequencing of 5hmC-enriched DNA was performed in a small cohort of normal and OA chondrocytes to identify differentially hydroxymethylated regions (DhMRs) in OA chondrocytes. Data from the genome-wide sequencing of 5hmC-enriched DNA were intersected with global OA gene expression data to define subsets of genes and pathways potentially affected by increased 5hmC levels in OA chondrocytes.

Results

A t

SUBMITTER: Taylor SE 

PROVIDER: S-EPMC4519426 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature