Human Urine-Derived Renal Progenitors for Personalized Modeling of Genetic Kidney Disorders.

ABSTRACT: The critical role of genetic and epigenetic factors in the pathogenesis of kidney disorders is gradually becoming clear, and the need for disease models that recapitulate human kidney disorders in a personalized manner is paramount. In this study, we describe a method to select and amplify renal progenitor cultures from the urine of patients with kidney disorders. Urine-derived human renal progenitors exhibited phenotype and functional properties identical to those purified from kidney tissue, including the capacity to differentiate into tubular cells and podocytes, as demonstrated by confocal microscopy, Western blot analysis of podocyte-specific proteins, and scanning electron microscopy. Lineage tracing studies performed with conditional transgenic mice, in which podocytes are irreversi

SUBMITTER: Lazzeri E 

PROVIDER: S-EPMC4520157 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature