Neoplastic transformation of porcine mammary epithelial cells in vitro and tumor formation in vivo.

ABSTRACT:

Background

The mammary glands of pigs share many functional and morphological similarities with the breasts of humans, raising the potential of their utility for research into the mechanisms underlying normal mammary function and breast carcinogenesis. Here we sought to establish a model for the efficient manipulation and transformation of porcine mammary epithelial cells (pMEC) in vitro and tumor growth in vivo.

Methods

We utilized a vector encoding the red florescent protein tdTomato to transduce populations of pMEC from Yorkshire -Hampshire crossbred female pigs in vitro and in vivo. Populations of primary pMEC were then separated by FACS using markers to distinguish epithelial cells (CD140a-) from stromal cells (CD140a+), with or without further enrichment for basal and

SUBMITTER: Rowson-Hodel AR 

PROVIDER: S-EPMC4520266 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature