Birc7: A Late Fiber Gene of the Crystalline Lens.

ABSTRACT:

Purpose

A distinct subset of genes, so-called "late fiber genes," is expressed in cells bordering the central, organelle-free zone (OFZ) of the lens. The purpose of this study was to identify additional members of this group.

Methods

Fiber cells were harvested from various layers of the lens by laser micro-dissection and subjected to microarray, in situ hybridization, and Western blot analysis.

Results

Expression of Livin, a member of the inhibitor of apoptosis protein (IAP) family encoded by Birc7, was strongly upregulated in deep cortical fiber cells. The depth-dependent distribution of Livin mRNA was confirmed by quantitative PCR and in situ hybridization. The onset of Livin expression coincided with loss of organelles from primary fiber cells. Livin expression pea

SUBMITTER: De Maria A 

PROVIDER: S-EPMC4520384 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature