Identification and functional analysis of early gene expression induced by circadian light-resetting in Drosophila.

ABSTRACT:

Background

The environmental light-dark cycle is the dominant cue that maintains 24-h biological rhythms in multicellular organisms. In Drosophila, light entrainment is mediated by the photosensitive protein CRYPTOCHROME, but the role and extent of transcription regulation in light resetting of the dipteran clock is yet unknown. Given the broad transcriptional changes in response to light previously identified in mammals, we have sought to analyse light-induced global transcriptional changes in the fly's head by using Affymetrix microarrays. Flies were subjected to a 30-min light pulse during the early night (3 h after lights-off), a stimulus which causes a substantial phase delay of the circadian rhythm. We then analysed changes in gene expression 1 h after the light stimulus.

SUBMITTER: Adewoye AB 

PROVIDER: S-EPMC4521455 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature