Extracellular vesicle-mediated phenotype switching in malignant and non-malignant colon cells.

ABSTRACT:

Background

Extracellular vesicles (EVs) are secreted from many cells, carrying cargoes including proteins and nucleic acids. Research has shown that EVs play a role in a variety of biological processes including immunity, bone formation and recently they have been implicated in promotion of a metastatic phenotype.

Methods

EVs were isolated from HCT116 colon cancer cells, 1459 non-malignant colon fibroblast cells, and tumor and normal colon tissue from a patient sample. Co-cultures were performed with 1459 cells and malignant vesicles, as well as HCT116 cells and non-malignant vesicles. Malignant phenotype was measured using soft agar colony formation assay. Co-cultures were also analyzed for protein levels using mass spectrometry. The importance of 14-3-3 zeta/delta in trans

SUBMITTER: Mulvey HE 

PROVIDER: S-EPMC4522096 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature