Ontology highlight
ABSTRACT:
SUBMITTER: Stummer D
PROVIDER: S-EPMC4522179 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Stummer Daniela D Herrmann Carmen C Rentmeister Andrea A
ChemistryOpen 20150204 3
Bioorthogonal click reactions are powerful tools to specifically label biomolecules in living cells. Considerable progress has been made in site-specific labeling of proteins and glycans in complex biological systems, but equivalent methods for mRNAs are rare. We present a chemo-enzymatic approach to label the 5' cap of eukaryotic mRNAs using a bioorthogonal photoclick reaction. Herein, the N7-methylated guanosine of the 5' cap is enzymatically equipped with an allyl group using a variant of the ...[more]