Lipid-free Apolipoprotein A-I Structure: Insights into HDL Formation and Atherosclerosis Development.

ABSTRACT: Apolipoprotein A-I is the major protein in high-density lipoprotein (HDL) and plays an important role during the process of reverse cholesterol transport (RCT). Knowledge of the high-resolution structure of full-length apoA-I is vital for a molecular understanding of the function of HDL at the various steps of the RCT pathway. Due to the flexible nature of apoA-I and aggregation properties, the structure of full-length lipid-free apoA-I has evaded description for over three decades. Sequence analysis of apoA-I suggested that the amphipathic α-helix is the structural motif of exchangeable apolipoprotein, and NMR, X-ray and MD simulation studies have confirmed this. Different laboratories have used different methods to probe the secondary structure distribution and organization of both the l

SUBMITTER: Mei X 

PROVIDER: S-EPMC4522339 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature