Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation.

ABSTRACT: The alteration of cellular functions by anchoring macromolecules to specified organelles may reveal a new area of therapeutic potential and clinical treatment. In this work, a unique phenotype was evoked by influencing cellular behavior through the modification of subcellular structures with genetically targetable macromolecules. These fluorogen-functionalized polymers, prepared via controlled radical polymerization, were capable of exclusively decorating actin, cytoplasmic, or nuclear compartments of living cells expressing localized fluorgen-activating proteins. The macromolecular fluorogens were optimized by establishing critical polymer architecture-biophysical property relationships which impacted binding rates, binding affinities, and the level of internalization. Specific labeling o

SUBMITTER: Magenau AJ 

PROVIDER: S-EPMC4522379 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature