Bone marrow-derived stromal cells are associated with gastric cancer progression.

ABSTRACT:

Background

The aim of this study was to clarify the role of bone marrow-derived stromal cells (BM-SCs) expressing CD271 in the development of gastric cancer.

Methods

The effect of human BM-SCs on the proliferation and motility of six gastric cancer cell lines, OCUM-2M, OCUM-2MD3, OCUM-12, KATO-III, NUGC-3, and MKN-74, was examined. CD271 expression levels in BM-SCs were analysed by flow cytometry. We also generated a gastric tumour model by orthotopic inoculation of OCUM-2MLN cells in mice that had received transplantation of bone marrow from the CAG-EGFP mice. The correlation between the clinicopathological features of 279 primary gastric carcinomas and CD271 expression in tumour stroma was examined by immunohistochemistry.

Results

Numerous BM-SCs infiltrated the gas

SUBMITTER: Kasashima H 

PROVIDER: S-EPMC4522640 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature