Purification and characterization of an anticoagulant oligopeptide from Whitmania pigra Whitman.

ABSTRACT:

Background

Dried Whitmania pigra is used for the treatment of cardiovascular and cerebrovascular diseases in traditional Chinese medicine and hot water and alcohol extracts also have anticogulant activity. However, a lower molecular weight and more stable anticogulant is needed.

Objective

The objective of the following study is to purify and characterize of an anticoagulant oligopeptide from Hirudo (Whitmania pigra Whitman).

Materials and methods

Gel filtration on Sephadex G-50, ion exchange on diethylaminoethyl-cellulose, and semi-prepared high-performance liquid chromatography were used to purify Hirudo. Automated coagulation analyzer was used for evaluating anticoagulant activity. Molecular weight was measured by Matrix-assisted laser desorption ionization time of

SUBMITTER: Zheng X 

PROVIDER: S-EPMC4522828 | biostudies-literature | 2015 Jul-Sep

REPOSITORIES: biostudies-literature