MicroRNA Expression Profiling of Propofol-Treated Developing Rat Hippocampal Astrocytes.

ABSTRACT: Although propofol exerts toxic effects on the developing central nervous system (CNS), it remains a first-choice anesthetic in the pediatric population. Astrocytes represent a major glial cell population whose role in CNS development is widely appreciated and that has been recently shown to be mediated in large part by microRNAs (miRNAs). In contrast, relatively little is known about the roles of miRNAs in developing astrocytes during propofol treatment. Here, miRNA microarray was used to profile fluctuations in miRNA expression in immature hippocampal astrocytes in response to propofol treatment, and results were subsequently validated using quantitative real-time polymerase chain reaction. Predictive analysis of genes targeted by propofol-regulated miRNAs indicated enrichment of genes in

SUBMITTER: Sun W 

PROVIDER: S-EPMC4523044 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature