Automated Scalable Heat Shock Modification for Standard Aquatic Housing Systems.

ABSTRACT: Heat shock is a common technique for inducible gene expression system in a variety of organisms. Heat shock treatment of adult zebrafish is more involved and generally consists of manually transferring fish between housing rack tanks and preheated water tanks or the use of timed heaters in stand-alone aquaria. To avoid excessive fish handling and to take advantage of the continuous flow of a standard housing rack, proposed modifications consisted of installing an aquarium heater inside each tank, manually setting the heater to reach heat shocking temperatures (> 37°C) and, after that, testing that every tank responded equally. To address the limitations in the existing systems, we developed a novel modification of standard zebrafish housing racks to perform heat shock treatment in conditio

SUBMITTER: Saera-Vila A 

PROVIDER: S-EPMC4523100 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature