Bisecting Galactose as a Feature of N-Glycans of Wild-type and Mutant Caenorhabditis elegans.

ABSTRACT: The N-glycosylation of the model nematode Caenorhabditis elegans has proven to be highly variable and rather complex; it is an example to contradict the existing impression that "simple" organisms possess also a rather simple glycomic capacity. In previous studies in a number of laboratories, N-glycans with up to four fucose residues have been detected. However, although the linkage of three fucose residues to the N,N'-diacetylchitobiosyl core has been proven by structural and enzymatic analyses, the nature of the fourth fucose has remained uncertain. By constructing a triple mutant with deletions in the three genes responsible for core fucosylation (fut-1, fut-6 and fut-8), we have produced a nematode strain lacking products of these enzymes, but still retaining maximally one fucose resid

SUBMITTER: Yan S 

PROVIDER: S-EPMC4523199 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature