Dynasore enhances the formation of mitochondrial antiviral signalling aggregates and endocytosis-independent NF-κB activation.

ABSTRACT:

Background and purpose

Dynasore has been used extensively as an inhibitor of clathrin-mediated endocytosis. While studying the role of endocytosis in LPS-induced signalling events, we discovered that dynasore itself induced activation of NF-κB, independently of its effects on endocytosis and without involving the Toll-like receptor 4 signalling pathways. The purpose of this study was to characterize this novel effect and to explore the underlying mechanism of action.

Experimental approach

We utilized gel electrophoresis, microscopy, gene knockdown and luciferase-based promoter activity to evaluate the effect of dynasore on cell signalling pathways and to delineate the mechanisms involved in its effects,

Key results

Dynasore activated the NF-κB and IFN-β pathways by ac

SUBMITTER: Ailenberg M 

PROVIDER: S-EPMC4523333 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature