The atypical antipsychotics clozapine and olanzapine promote down-regulation and display functional selectivity at human 5-HT7 receptors.

ABSTRACT:

Background and purpose

Classically, ligands of GPCRs have been classified primarily upon their affinity and efficacy to activate a signal transduction pathway. Recent reports indicate that the efficacy of a particular ligand can vary depending on the receptor-mediated response measured (e.g. activating G proteins, other downstream responses, internalization). Previously, we reported that inverse agonists induce both homo- and heterologous desensitization, similar to agonist stimulation, at the Gs -coupled 5-HT7 receptor. The primary objective of this study was to determine whether different inverse agonists at the 5-HT7 receptor also induce internalization and/or degradation of 5-HT7 receptors.

Experimental approach

HEK293 cells expressing 5-HT7(a, b or d) receptors were pre

SUBMITTER: Andressen KW 

PROVIDER: S-EPMC4523340 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature