In utero localized diffusion MRI of the embryonic mouse brain microstructure and injury.

ABSTRACT:

Purpose

To develop an in vivo diffusion magnetic resonance imaging (dMRI) technique to study embryonic mouse brain structure and injury.

Materials and methods

Pregnant CD-1 mice were examined on embryonic day 17 on an 11.7T scanner. Spatially selective excitation pulses were used to achieve localized imaging of individual mouse brains, in combination with a 3D fast imaging sequence to acquire dMRI at 0.16-0.2 mm isotropic resolution. Subject motions were corrected by navigator echoes and image registration. Further acceleration was achieved by simultaneous imaging of two embryos in an interleaved fashion. We applied this technique to detect embryonic brain injury in a mouse model of intrauterine inflammation.

Results

With the localized imaging technique, we achieved i

SUBMITTER: Wu D 

PROVIDER: S-EPMC4523472 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature